histological study of the hoof development in sheep

in orde r to study of the hoof development in sheep . 16 healthy sheep embryos with different ages immediately after slaughters of their mothers in slaughter house were collected hooges were cut half sagitilly and fixed in 10% buffered formalin.routine histologic processes were used and 6µ thick paraffin sections were stained by haematoxylin-eosin method and studied under light microscope.the hoof has covered by the real skin at first. the primary changes will be seen on the stratum germinatvum of the hoof skin, which caused to formation of the tunica interna of the wall and the papillary layer of the sole and heel. the more supperficial layers were then made by them, so that the tunica media of the wall were made by the epidermis of the corium and upper of the wall. it made up of hard keratin, thus the manufacturing epidermis of this layer were devoided of the stratum granulosum and keratohyalin granules.the tubular layer of the white line were made by the lower epidermis of the wall. the papillay layers of other parts of the hoof were made by the papillary epidermis of the same parts. the last layer were made before birth was the tunica externa. this layer were made by the papillay epidermis of the periople skin.